{"created":"2023-07-07T04:34:39.159610+00:00","id":2000005,"links":{},"metadata":{"_buckets":{"deposit":"48048d00-525a-4560-90ae-0a069e761b75"},"_deposit":{"created_by":8,"id":"2000005","owner":"8","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2000005"},"status":"published"},"_oai":{"id":"oai:nuhm.repo.nii.ac.jp:02000005","sets":["1:1688519611790"]},"author_link":[],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-01"},"bibliographicPageEnd":"39","bibliographicPageStart":"25","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"長野保健医療大学紀要","bibliographic_titleLang":"ja"}]}]},"item_10002_description_4":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_description":"NUHM043","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ホスホフルクトキナーゼ（6- ホスホフルクト 1- キナーゼ、PFK-1、EC 2.7.1.11）は解糖系の律速酵素である。PFK-1 が律速酵素であるのは、様々な調節因子の細胞内濃度の変化によって、その活性が調節されるためである。特に肝臓では、栄養状態の変動によるインスリンやグルカゴンなどのホルモンに導かれた調節を受ける。研究の初期には AMP、フルクトース 6- リン酸、フルクトース 1,6-ビスリン酸による活性化、ATP やクエン酸による阻害など、糖代謝の代謝中間体による調節が明らかにされた。続いて代謝中間体ではない、解糖系の活性化のみに働くフルクトース 2,6- ビスリン酸（Fru2,6-P2）が発見された。その後、低酸素状態により極めて短時間で急激に増加する活性化因子である、リボース 1,5- ビスリン酸（Rib-1,5-P2）の産生が脳で見いだされ、次いでマクロファージで示された。Fru-2,6-P2 は多くの組織で産生と分解にかかわる酵素の存在が示され、その活性調節機構はアイソザイムにより異なるが、低酸素刺激や栄養状態の変化による複数のプロテインキナーゼ、ホスファターゼの活性化を介してリン酸化・脱リン酸化により調節される。一方 Rib-1,5-P2 は低酸素刺激により脳では 5 秒以内、マクロファージでは 30 秒をピークとして急激に産生され、PFK-1 を素早く活性化するため、解糖系活性化のトリガーとしての役割が示唆されている。Rib-1,5-P2 の産生にかかわる酵素は、マクロファージにおいてリン酸化による活性化が示されたが、いまだ不明な点が多い。この総説では、PFK-1 の活性化にかかわる因子のうち、主に Fru-2,6-P2 と Rib-1,5-P2 について解説する。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Phosphofructokinase (6-phosphofructo-1-kinase, PFK-1, EC 2.7.1.11) is a rate-limiting enzyme in glycolysis. PFK-1 is a rate-limiting enzyme because changes in the intracellular concentrations of various regulatory factors regulate its activity. The liver, in particular, is subject to hormone-induced regulation, such as insulin and glucagon, due to fluctuations in nutritional status. Early on, regulation by intermediate metabolites of glucose metabolism was known, including activation by AMP, fructose 6-phosphate, fructose 1,6-bisphosphate, and inhibition by ATP and citrate. Subsequently, activation by fructose 2,6-bisphosphate (Fru-2,6-P2), which is not an intermediate metabolite but only activates the glycolytic system, was clarified. Later, the production of Ribose 1,5-bisphosphate (Rib-1,5-P2), an activator that rapidly increases and quickly disappears upon hypoxia, was found in the brain, followed by macrophages. Fru-2,6-P2 is known to be produced by enzymes involved in its synthesis and degradation in many tissues. Fru-2,6-P2 has been shown to be made and degraded by enzymes in many tissues. Its activity is regulated by phosphorylation and dephosphorylation via the activation of multiple protein kinases and phosphatases by hypoxic stimuli and changes in nutritional status. However, the regulatory mechanism differs among isoenzymes. On the other hand, Rib-1,5-P2 is rapidly produced by hypoxic stimuli within 5 seconds in the brain and peaking at 30 seconds in macrophages. It rapidly activates PFK-1, suggesting its role as a trigger for the activation of the glycolytic system. The enzyme involved in the production of Rib-1,5-P2 has been shown to be activated by phosphorylation in macrophages, but much remains unknown. This review will focus mainly on Fru-2,6-P2 and Rib-1,5-P2 among the factors involved in PFK-1 activation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20841/0002000005","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長野保健医療大学","subitem_publisher_language":"ja"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三井 由香","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-07-07"}],"displaytype":"detail","filename":"長野保健医療大学紀要第8巻_25-39.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_5","url":{"url":"https://nuhm.repo.nii.ac.jp/record/2000005/files/長野保健医療大学紀要第8巻_25-39.pdf"},"version_id":"cd2d1385-08dc-4b65-bc03-cabd9fa8ff31"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ホスホフルクトキナーゼ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"フルクトース 2,6- ビスリン酸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"リボース 1,5- ビスリン酸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphofructokinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fructose 2,6-bisphosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ribose 1,5-bisphosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ホスホフルクトキナーゼの活性調節 ―フルクトース 2,6-ビスリン酸とリボース 1,5-ビスリン酸を主点として―","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ホスホフルクトキナーゼの活性調節 ―フルクトース 2,6-ビスリン酸とリボース 1,5-ビスリン酸を主点として―","subitem_title_language":"ja"},{"subitem_title":"Regulation of phosphofructokinase activation:   Focusing on fructose 2,6-bisphosphate and ribose 1,5-bisphosphate","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"8","path":["1688519611790"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-07-07"},"publish_date":"2023-07-07","publish_status":"0","recid":"2000005","relation_version_is_last":true,"title":["ホスホフルクトキナーゼの活性調節 ―フルクトース 2,6-ビスリン酸とリボース 1,5-ビスリン酸を主点として―"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2023-07-07T04:54:17.265321+00:00"}